LV-EF1α-FLPo-Puro; LV-EF1A-FLPo-Puro; LV-EF1α-FLPo-IRES-Puro; EF1A-FLPo-Puro Lentivirus; EF1α-FLPo-Puro Lentivirus
Product Category:
Pre-made recombinant lentivirus; LV-EF1α-FLPo-Puro;
LV-EF1A-FLPo-Puro; LV-EF1α-FLPo-IRES-Puro;
EF1A-FLPo-Puro Lentivirus; EF1α-FLPo-Puro
Lentivirus
Description: FLP
originally identified from Saccharomyces cerevisiae is a site-specific
recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT
sequence. It has been widely used for generating conditional knockout mice,
mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed
inefficient recombinase activity due to thermo-instability of the FLP protein.
Subsequent screening for thermo-stable mutants resulted in the identification of
FLPe which was 4-fold increase in recombination efficiency at 37 °C.
However the recombination efficiency of FLPe in cells remains very low because
of its non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT
recombination efficiency for both in vivo and in vitro in mouse.
This LV-EF1α-FLPo-Puro
pre-made lentivirus expresses
FLPo recombinase with co-expression of puromycin selection in an IRES cassette
under EF1α
promoter. Ready to use format.
Titer:
> 1x109 TU/mL
Selection Antibiotic: Puromycin
Purity*: In
vivo grade and OK for both in vitro tissue culture infection and in vivo
injection
Size: 2 x
25 µL (Std Pack)
Storage Buffer:
PBS
Infection Protocol:
Transfer Vector Map:
*
Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm
