Price: $468.00

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  • Catalog #: SL100300
  • Unit Size: 2 x 25 ÁL (>10^9 TU/mL)

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LV-CMV-FLPo-Puro; LV-CMV-FLPo-IRES-Puro; CMV-FLPo-Puro Lentivirus.

Product Category: Pre-made recombinant lentivirus; LV-CMV-FLPo-Puro; LV-CMV-FLPo-IRES-Puro; CMV-FLPo-Puro Lentivirus.

Description: FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 ░C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. 

FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse.
This LV-CMV-FLPo-Puro pre-made lentivirus expresses FLPo recombinase with co-expression of puromycin selection in an IRES cassette under CMV promoter. Ready to use format.

Titer: > 1x109 TU/mL

Selection Antibiotic: Puromycin

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 ÁL (Std Pack)

Storage Buffer: PBS

Infection Protocol:

Transfer Vector Map:
LV-CMV-FLPo-Puro; LV-CMV-FLPo-IRES-Puro; CMV-FLPo-Puro Lentivirus.

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm

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