Product Category: human Adenovirus Type5 (dE1/E3). Ad-EYFP-Actin or Ad-CMV-EYFP-Actin
Description:
This recombinant human adenovirus
type 5 expresses EYFP-Actin which encodes a fusion of the enhanced yellow
fluorescent protein (EYFP) and human cytoplasmic beta-actin. EYFP is an
enhanced yellow-green variant of the Aequorea victoria green fluorescent
protein (GFP). The EYFP gene contains the four amino acid substitutions
previously published as GFP-10C: Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala;
and Thr-203 to Tyr. The fluorescence excitation maximum of EYFP is 513 nm;
the emission spectrum has a peak at 527 nm. This control adenovirus will
eventually infect all mammalian cells and will be used to visualize the network
of microfilaments.
Titer: 1x1010 ~ 1x1011 PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
User Manual:
Shuttle Vector Map:
