Pre-made recombinant AAV; AAV-Synapsin-iCre; AAV(PHP.eB)-Synapsin-iCre; AAV-Syn-Cre; AAV(PHP.eB)-Syn-iCre
Product
Category: Pre-made recombinant AAV; AAV-Synapsin-iCre;
AAV(PHP.eB)-Synapsin-iCre; AAV-Syn-Cre; AAV(PHP.eB)-Syn-iCre
Description:
Cre
recombinase is a type I topoisomerase from bacteriophage P1 that catalyzes the
site-specific recombination of DNA between loxP sites. Cre recombinase is used
as a tool to genetically modify genes, such as to delete a segment of DNA
flanked by LoxP sites in cells or experimental animals. By optimizing the
mammalian codon usage for Cre recombinase, Cre expression is improved in the
mammalian cells. This codon-improved Cre (known as iCre) transgene shows lower
CpG content from the prokaryotic coding sequence, thus reducing the chances of
epigenetic silencing in mammals.
AAV(PHP.eB)-Synapsin-iCre
is a
pre-packaged rAAV in serotype PHP.eB (with capsid from AAV serotype PHP.eB and
2xITR from AAV serotype 2) which over-expresses Cre recombinase under human
synapsin promoter for exclusive neuronal expression. This product used in the
Cre-lox system as a genetic tool to generate site-specific recombination of DNA
between loxP sites in cultured cells and animal experiments. Compared
with rAAV serotype PHP.B, rAAV in serotype PHP.eB is able to penetrate
brain-blood barrier (BBB) and infect CNS via tail vein I.V. with 5 times better
transduction efficiency. Ready to use format.
Titer: >
1x1013 VG/mL
Size: >30
µL(Std Pack)
Storage Buffer: Sterile
PBS
Infection Protocol: 
Vector Map:
