Product 349/467
Product Category: Pre-made recombinant AAV | AAV-hSynapsin-DIO-tdTomato | AAV-Synapsin-DIO-tdTomato | AAV9-hSyn-DIO-tdTomato | AAV9-Syn-DIO-tdTomato Description: AAV9-Syn-DIO-tdTomato is a pre-made rAAV in serotype 9 which will Cre dependently over-express tdTomato driven under human synapsin promoter as illustrated as the cartoon below. Tandem dimer tomato (tdTomato) is an exceptionally bright red fluorescent protein with about 6x brighter than EGFP and has been used in a variety of applications. It is highly photostable and resistant to photobleaching. In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5' promoter and is flanked by oppositely oriented loxP and lox2272 sites. In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be "Flip-exchanged" or FLEXed, leading to the expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV). Titer: > 1x1013 VG/mL Size: 30 µL (Std Pack) Storage Buffer: Sterile PBS Infection Protocol: Vector Map:
