Pre-made recombinant AAV; AAV-CAG-Gluc; AAV1-CAG-Gluc; AAV-CAG-fGluc; AAV1-CAG-fGluc
Product
Category:
Pre-made recombinant
AAV; AAV-CAG-Gluc; AAV1-CAG-Gluc; AAV-CAG-fGluc; AAV1-CAG-fGluc
Description:
AAV1-CAG-fGluc is a pre-packaged rAAV in serotype 1 (capsid from AAV
serotype 1 and 2xITR from
AAV serotype 2) which over-expresses
humanized form of Gaussia luciferase (hGluc) under CAG
(also known as CBA promoter).
The humanized form of Gaussia luciferase
(hGLuc) was confirmed to be nontoxic and naturally secreted after
over-expression in mammalian cells. hGLuc generated over 1000-fold higher
bioluminescent signal intensity from live cell and over 100-fold higher
intensity from viable cells alone (not including secreted luciferase) or cell
lysates, compared to humanized forms of firefly (hFLuc) and Renilla (hRLuc)
luciferases expressed under similar conditions. Furthermore, hGLuc showed
200-fold higher signal intensity than hRLuc and intensity comparable to that of
hFLuc in vivo under standard imaging conditions. Gaussia luciferase provides a
sensitive means of imaging gene delivery and other events in living cells in
both tissue culture and in vivo, with a unique combination of features including
high signal intensity, secretion, and ATP independence, thus being able to
report from the cells and their environment in real time. Ready to use format.
Titer:
> 1x1013 VG/mL
Size:
30 µL (Std
Pack)
Storage Buffer:
PBS
Infection Protocol:
Vector Map:
