shRNA/miRNA Validation Service

shRNA/miRNA Validation Service via Cell-based Reporter System:
- Cloning ORF of gene of interests (GOI) into a rAAV cis vector with a reporter.
- Design and synthesize ~6 shRNA oligoes followed by cloning them to the above rAAV cis construct to obtain shRNA validation plasmids.
- For miRNA validation, synthesize pre-miRNA oligoes followed by cloning them to the above rAAV cis construct to obtain shRNA validation plasmid.
- Transient transfection the shRNA/miRNA validation plasmids.
- Quantification of target gene silencing via FACS (for GFP expression) or luminometer (for luciferase expression).

Available shRNA/miRNA Validation Platforms:
- Constitutive shRNA knockdown
- Inducible shRNA knockdown

Guaranteed Knockdown:
We guarantee at least 1 (one) shRNA with >80% knockdown of mRNA level in the tested cells by screening ~6 shRNA/miRNA oligoes.

We offer discount for new customer, please  request a quote with us today.
 

Figure 1.  A Cartoon Showing shRNA Validation Process for shRNA rAAV Production.