shRNA/miRNA/gRNA Adenovirus Production Full Service Pilot Scale
Production of Recombinant Serotype 5 shRNA/miRNA Adenovirus (E1/E3 deletion) with Ad.MAX System:
- One-stop service: from shRNA/miRNA design to packaging.
- Unsurpassable transduction efficiency.
- Quick turnaround time: 3 to 4 weeks.
- Cost-effective.
- Special design request acceptable, FREE!
Large shRNA/miRNA/gRNA Ad.MAX™ shuttle vector collection for cloning shuttle plasmid with a specific promoter and reporter:
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Single-promoter Ad.MAX™ shuttle vector
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Dual-promoter Ad.MAX™ shuttle vector
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Promoterless
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
ALB(1.4)
ApoE/AAT1
CaMKII
ELA1
Enh358MCK
cTNT
GFAP
MBP
SST
TBG
αMHC
hRPE
mIP1
tMCK
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Promoterless
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
ALB(1.4)
ApoE/AAT1
CaMKII
ELA1
Enh358MCK
cTNT
GFAP
MBP
SST
TBG
αMHC
hRPE
mIP1
tMCK
|
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
CaMKII
cTNT
GFAP
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eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ
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Service Description:
1. Synthesize and clone shRNA/miRNA to a shuttle vector followed by transfer of the whole transgene cassette to adenoviral genome.
2. Viral production in Ad.MAX™ 293 cells: transfection and purification of recombinant adenovirus.
3. Generation of high titer recombinant adenoviral stock in ~500 cm2 Ad.MAX™ 293 cells.
4. Recombinant adenovirus PFU titration.
Required Materials: NCBI accession # of target gene, target region sequence, shRNA or siRNA sequence validated/published or mature miRNA sequence, miRNA accession # or gRNA sequence etc.
Turnaround Time: 3 ~ 4 weeks.
Deliverables: 1.0 mL (5x0.2 mL) of super infectious in vitro grade adenovirus vector at 5E+10~5E+11 PFU/mL*.
We offer discount for new customer, please request a quote with us today.
We also offer truncated custom shRNA/miRNA/gRNA adenovirus service, please request a quote with us today.
* Final viral yield may depend on the transgene in the virus.
