GenJet™ In Vitro DNA Transfection Reagent for NMuMG Cells

Price: $156.00

Add to Cart:

  • Catalog #: SL100489-NMuMG
  • Unit Size: 1.0 ml

Please Choose:



Description

GenJet™ DNA In Vitro Transfection Reagent for NMuMG cells is pre-optimized for transfecting NMuMG cells. NMuMG is mouse mammary gland, normal epithelial. NMuMG cells express different integral surface proteoglycans (e.g. syndecan). They are used for studies of cell adhesion and growth factor-binding molecules as well as for analysis of growth factor effects on cell proliferation.

Refer to the following optimal transfection conditions for maximal transfection efficiency on NMuMG cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~90%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
No
48 hours


EGFP
pEGFP-N3 (CMV promoter)

75%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on NMuMG Cells. NMuMG cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection

Data Sheet




Copyright © 2017 SignaGen Laboratories. All Rights Reserved.