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Concentrate Lentivirus by Ultra-centrifugation

Ultracentrifuge: Beckman LE 80K Rotor: SW28 Tubes: #326823 autoclaved (polyallomer) 1. Pool all viral media into and filter through a 0.45μm HV Durapore membrane (Millipore #SCHVU01RE). 2. Place tubes into […]

Lentivirus Purification by Ultra-centrifugation

Before Starting: Required equipments and consumbles: 1. SW-28 rotor with buckets and caps (Kept in 4C Refrigerator in Centrifuge Room) 2. SW-28 thin walled ultra clear centrifuge tubes (Beckman # […]

Formulation of Lipofectamine 2000 and Lipofectin

LIPOFECTIN is a 1:1 (w/w) mixture of N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) and dioleoylphosphatidylethanolamine (DOPE). LIPOFECTAMINE is a 3:1 (w/w) mixture of the polycationic lipid, 2,3-dioleyloxy-N-[2(sperminecarboxamido)ethyl]-N,N-dimethyl-1-propanaminium trifluoroacetate (DOSPA), and DOPE.

Lentivirus Titration via qRT-PCR

The transduction unit (TU or IFU) of the lentiviral particles was estimated using the formula which 1TU=100 copies of viral genomic RNA. The physical copy numbers of the viral genomic […]