Most current methods of gene delivery for primary cultured hippocampal neurons are limited by toxicity, transient expression, the use of immature neurons, and/or low efficiency. We performed a direct comparison […]
Differential Effects of AAV-BDNF and AAV-NTF3 in the Deafened Adult Guinea Pig Ear
Intracochlear neomycin administration resulted in a complete loss of hair cells and supporting cells in the basal turn, forming a flat epithelium. A few looping auditory fibers are seen in […]
A Novel Synthetic AAV Capsid 8BP2 for Efficient Transduction of Retinal Bipolar Cells
The map of AAV serotype 8BP2
Transfection Techniques for Neuronal Cells
Summary of advantages and disadvantages of different techniques commonly used to transfect mammalian neurons Best suited for Strengths Limitations Toxicity Onset, level, and duration of expressiona Maximum insert size Genome […]
Full Sequence of Ace2N-4AA-mNeon, A Novel Calcium Sensor
atggctgacgtggaaaccgagaccggcatgattgcacagtggattgtctttgctattatggctgctgctgctattgcttttggagtggctgtgcactttcggccttcagagctgaagagcgcatactatatcaac attgccatctgcactatcgccgctaccgcttactatgcaatggccgtgaactaccaggacctgacaatgaatggtgaaaggcaggtggtctacgcaagatatattaactgggtgctgaccacaccactgctcctg ctcgatctcatcgtcatgaccaagatgggcggagtgatgatttcttgggtcatcggcgcagacattttcatgatcgtgtttggtattctgggcgccttcgaggatgaacacaagttcaaatgggtgtactttatcg ctggatgtgtgatgcaggcagtcctgacatacgggatgtataacgccacttggaaagacgatctgaagaaaagccccgagtaccatagctcctatgtcagtctgctcgtcttcctgtcaatcctctgggtgtttt atcctgtcgtgtgggctttcgggtctggtagtggcgtgctgtccgtcgacaatgaggccattctcatgggaatcctggatgtgctcgctaagccactgtttggaatggggtgcctcattgcccatgagactatctt caagaagatgctgaggtctctcccagcgacacatgagttacacatctttggctccatcaacggtgtggactttgacatggtgggtcagggcaccggcaatccaaatgatggttatgaggagttaaacctgaagt ccaccaagggtgacctccagttctccccctggattctggtccctcatatcgggtatggcttccatcagtacctgccctaccctgacgggatgtcgcctttccaggccgccatggtagatggctccggataccaagtcc atcgcacaatgcagtttgaagatggtgcctcccttactgttaactaccgctacacctacgagggaagccacatcaaaggagaggcccaggtgaaggggactggtttccctgctgacggtcctgtgatgaccaact cgctgaccgctgcggactggtgcaggtcgaagaagacttaccccaacgacaaaaccatcatcagtacctttaagtggagttacaccactggaaatggcaagcgctacaggagcactgcgcggaccacctacac ctttgccaagccaatggcggctaactatctgaagaaccagccgatgtacgtgttccgtaagacggagctcaagcactccaagaccgagctcaacttcaaggagtggcaaaaggcctttaccgatgtgatgggcat ggacgagctgtacaagaagagcaggatcaccagcgagggcgagtacatccccctggaccagatcgacatcaacgtgttctgctacgagaacgaggtgtaatga
AAV–BDNF mediated attenuation of quinolinic acid-induced neuropathology and motor function impairment
Maintenance and plasticity of striatal neurons is dependant on brain-derived neurotrophic factor (BDNF), which is depleted in the Huntington’s disease striatum due to reduced expression and disrupted corticostriatal transportation. In […]
mIFP Full Sequence
atgtcggtaccgctgactacctcagcattcggccacgcgtttctggctaactgtgaacgcgagcagatccacctggcgggctccattcagccgcac ggtatcctgctggctgtgaaagagccggacaacgtggtgatccaggcttctattaacgctgcggagttcctgaacaccaactctgttgttggcc gtccgctgcgtgacctgggcggcgatctgcctttgcagatcctgccgcacctgaacggcccgctgcacctggctccgatgaccctgcgttgtacc gtgggttctccgccgcgtcgtgtggactgtaccattcatcgtccgtctaacggcggcctgatcgtagaactggaaccagcaaccaagaccact aacattgcgccggctctggacggtgcgtttcatcgtatcacttcttcatcctccctgatgggcctgtgtgacgaaaccgcgactattatccgtgaga ttactggctacgaccgtgtgatggtagtacgtttcgatgaagagggtaatggcgaaattctgtccgaacgtcgtcgtgcggacctggaagcgtt cctgggtaaccgctacccggcgtctactattccgcagatcgctcgtcgcctgtacgaacataaccgtgttcgcctgctggtagatgtgaactat actccggttccgctacagccgcgcatcagcccgctgaacggtcgtgatctggatatgtccctgtcttgcctgcgctctatgtccccgatccacca gaaatacatgcaggacatgggcgttggcgcgaccctggtttgctctctgatggtgtctggtcgtctgtggggtctgatcgcttgccaccacta cgaaccgcgcttcgttccgttccacattcgcgctgctggcgaagcgctggcggaaacttgtgcgatccgcatcgcgacgctggagagcttt gcacagtctcagtccaaatga
Infrared fluorescent proteins
Hunting for new fluorescent proteins in the coral reefs of the Caribbean and Australia is a task that a lucky few researchers have managed to get funding for. Scuba diving […]