A quantitative, DNA-based, real-time PCR approach to determine the number of lentivirus particles that are present in vector preparations was reported. In this approach, the minus strong-stop cDNA fragment that is present in viral capsids serves as […]
Primers targeting minus strong-stop cDNA fragment for lentivirus titration
Forward: 5′-AGCTTGCCTTGAGTGCTTCA-3′ Reverse: 5′-TGACTAAAAGGGTCTGAGGGA-3′
Optimal MOIs for Some Cells Infected with Lentivirus
Cell line Tissue Cancer/cell type Species MOI A431 Epithelial Carcinoma Human 5 A549 Lung Carcinoma Human 5 Astrocytes Nervous system Primary Human 1 B16-F10 Epithelial Melanoma, metastatic Mouse 5 BMM Bone Marrow Primary Human 8 […]
What is the termination sequence for U6 promoter?
The termination sequence for the U6 promoter is a series of T’s (5-6) in a row.
What is the U6 promoter transcription start site?
The “G” that you mentioned is in fact the “preferred” transcription start site, however, this can be replaced by any other nucleotide. It’s just that U6 prefers “G” as the […]
Decoys and Tough Decoy (TuD) RNAs
Background Decoy and Tough Decoy (TuD) RNAs are novel technologies for regulating RNA interference (Haraguchi et al., Nucleic Acids Res. 2009; Xie et al., Nature 2012). In short, they are […]
Several shRNAs Validated
5′-GATCCCCGAAAGTACATTTTCAACTGTTCAAGAGACAGTTGAAAATGTACTTTCTTTTTGGAAA-3′ (HIPK2-1376 sense), 5′-AGCTTTTCCAAAAAGAAAAGTACATTTTCAACGTCTCTTGAACAGTTGAAAATGTACTTTCGGG-3′ (HIPK2-1376 antisense), 5′-GATCCCCGAACCACACGTGCTTGGTCTTCAAGAGAGACCAAGCACGTGTGGTTCTTTTTGGAAA-3′ (HIpk2-789 sense), and 5′-AGCTTTTCCAAAAAGAACCACACGTGCTTGGTCTCTCTTGAAGACCAAGCACGTGTGGTTCGGG-3′ (HIPK2-789 antisense). 5′-GATCCCCCAACAGGAGAGTCATTGTTTTCAAGAGAAACAATGACTCTCCTGTTGTTTTTGGAAA-3′ (Gal3-551, sense), 5′-AGCTTTTCCAAAAACAACAGGAGAGTCATTGTTTCTCTTGAAAACAATGACTCTCCTGTTGGGG-3′ (Gal3-551, antisense), 5′-GATCCCCACCTTACATGTGTAAAGGTTTCAAGAGAACCTTTACACATGTAAGGTTTTTTGGAAA-3′ (Gal3-845, sense), and 5′-AGCTTTTCCAAAAAACCTTACATGTGTAAAGGTTCTCTTGAAACCTTTACACATGTAAGGTGGG-3′ (Gal3-845, antisense).
Two shRNAs Targeting Mouse PDXP
Two shRNAs Targeting Mouse PDXP CCGGCAAGCCCAGCCCTTACATGTTCTCGAGAACATGTAAGGGCTGGGCTTGTTTTTG CCGGCTGGAGACCGACATACTCTTTCTCGAGAAAGAGTATGTCGGTCTCCAGTTTTTG
Target sequences for generation of ATG5 or ATG7 siRNAs
Target sequences for generation of ATG5 or ATG7 siRNAs siRNA construct Target sequences (5′–3′) atg5 Construct 1 CAACGTGCTTTACTCTCTA Construct 2 TGAGATAACTGAACGAGAA Construct 3 ATCTGAGCTATCCAGACAA Scrambled GTCCCGGATACCTAATAAA atg7 Construct 1 ACTACAATGGTGACTCTGC Construct 2 […]
Several Stealth Select siRNAs Target Region Sequences
Stealth Select siRNAs targeting ATG5 (no. 1; 5′-GGUUUGGACGAAUUCCAACUUGUUU-3′, no. 2; 5′-GAUCACAAGCAACUCUGGAUGGGAU-3′ and no. 3; 5′-GCCAUCAAUCGGAAACUCAUGGAAU-3′); BECN1 (no. 1; 5′-CCACUCUGUGAGGAAUGCACAGAUA-3′, no. 2; 5′-GGAUGAUGAGCUGAAGAGUGUUGAA-3′ and no. 3; 5′-ACAGUGAAUUUAAACGACAGCAGCU-3′); ATG7 (no. […]