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Eukaryotic Promoter Database

EPDnew (Eukaryotic Promoter Database) – is a new collection of experimentally validated promoters in human, mouse, D. melanogaster and zebrafish genomes.  Evidence comes from TSS-mapping from high-throughput expreriments such as […]

Common promoters for eukaryotes and prokaryotes

Eukaryotic Promoters   Promoter Primarily used for RNA     transcript Description Expression Additional considerations CMV General expression mRNA Stong mammalian expression promoter from the human cytomegalovirus Constitutive May contain an enhancer region. […]

Why EF1alpha promoter is so special?

Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. […]

DMTMM A Novel Coupling Reagent

Propylphosphonic anhydride (T3P®) is a very efficient, nontoxic coupling reagent especially suited for connecting sterically hindered amino acids. A further advantage is the easy removal of byproducts by extraction of the […]

Applications of Channelrhodopsin

Channelrhodopsins can be readily expressed in excitable cells such as neurons using a variety of transfection techniques (viral transfection, electroporation, gene gun). The light-absorbing pigment retinal is present in most […]

Discovery of Channelrhodopsin

Motility and photoorientation of microalgae have been studied over more than hundred years in many laboratories worldwide. In 1980, Ken Foster developed the first consistent theory about the functionality of […]