Alternatives to Fetal Bovine Serum in Cell Culture media, Challenges and Perspectives
Dr. Gerhard Gstraunthaler recently presented a seminar with the above title. Gerhard carries a faculty appointment with the Department of Physiology at Innsbruck Medical University, Austria. Gerhard is an advocate of serum-free […]
Role of Serum in Cell Culture Media
Serum provides > growth factors and hormones > binding and transport proteins > attachment and spreading factors > additional amino acids, vitamins and trace elements > fatty acids and lipids > […]
Lentivirus Production Protocol
Supplies: 293FT Cells (Invitrogen: R700-07) T-225 tissue culture flasks (Nunc: 159934) T-75 tissue culture flasks (Nunc: 156499) 500 cm2 tissue culture plates (Nunc: 166508) Ultracentrifuge tubes (Beckman Culture: 344058) DMEM […]
Plasmids for Optogenetics Sensors from Addgene
Addgene’s repository contains a variety of genetically encoded biosensors to monitor and measure fluctuations in molecular signals in cells, including those to monitor calcium, glutamate, pH, and voltage. These optogenetics […]
Optogenetics rAAVs
INTRSECT (INTronic Recombinase Sites Enabling Combinatorial Targeting)-related constructs A versatile single-AAV system for selective expression that is conditional upon multiple cell-type features (such as wiring or genetic type) related by […]
Fetal Bovine Serum – the Tipping Point
Users of US Fetal Bovine Serum have been watching for months and months how prices climb up along with a constantly growing shortage and with no end in sight. Yet […]
Fetal Bovine Serum Availability – How do Regulations Affect It?
Now we will talk about the influence of various regulations on the availability of Fetal Bovine Serum. It is a fact that import and export regulations have direct influence on […]
>100 Pre-made Ready to Use rAAVs from SignaGen
All the following pre-made AAVs were packaged in serotype 1**, 2, 5, 6, 8** and 9** and super purified via 2xCsCl ultra-centrifugation in in-vivo grade. All the pre-packaged rAAVs were […]
Summary of clinical trials using AAV gene transfer
Disease Transgene product Serotype Route of administration Clinical trial ClinicalTrials.gov identifier Refs AAV clinical trials for inherited diseases α1 antitrypsin deficiency α1 antitrypsin AAV2 Intramuscular Phase I/II NCT00377416 101,102 AAV1 […]