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AAV5 Complete Genome

1 ctctcccccc tgtcgcgttc gctcgctcgc tggctcgttt gggggggtgg cagctcaaag 61 agctgccaga cgacggccct ctggccgtcg cccccccaaa cgagccagcg agcgagcgaa 121 cgcgacaggg gggagagtgc cacactctca agcaaggggg ttttgtaagc agtgatgtca 181 taatgatgta atgcttattg tcacgcgata gttaatgatt aacagtcatg tgatgtgttt 241 tatccaatag […]

AAV2 Complete Genome

1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 61 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg 121 gccaactcca tcactagggg ttcctggagg ggtggagtcg tgacgtgaat tacgtcatag 181 ggttagggag gtcctgtatt agaggtcacg tgagtgtttt gcgacatttt gcgacaccat 241 gtggtcacgc […]

AAV2 Capsid Protein VP3

10 20 30 40 50 MATGSGAPMA DNNEGADGVG NSSGNWHCDS TWMGDRVITT STRTWALPTY 60 70 80 90 100 NNHLYKQISS QSGASNDNHY FGYSTPWGYF DFNRFHCHFS PRDWQRLINN 110 120 130 140 150 NWGFRPKRLN FKLFNIQVKE VTQNDGTTTI ANNLTSTVQV FTDSEYQLPY […]

AAV2 Capsid Protein VP2

10 20 30 40 50 MAPGKKRPVE HSPVEPDSSS GTGKAGQQPA RKRLNFGQTG DADSVPDPQP 60 70 80 90 100 LGQPPAAPSG LGTNTMATGS GAPMADNNEG ADGVGNSSGN WHCDSTWMGD 110 120 130 140 150 RVITTSTRTW ALPTYNNHLY KQISSQSGAS NDNHYFGYST PWGYFDFNRF […]

AAV2 Capsid Protein VP1

10 20 30 40 50 MAADGYLPDW LEDTLSEGIR QWWKLKPGPP PPKPAERHKD DSRGLVLPGY 60 70 80 90 100 KYLGPFNGLD KGEPVNEADA AALEHDKAYD RQLDSGDNPY LKYNHADAEF 110 120 130 140 150 QERLKEDTSF GGNLGRAVFQ AKKRVLEPLG LVEEPVKTAP GKKRPVEHSP […]

Capsid mutation(s) of rAAV leads to enhanced transduction efficiency

AAV Serotype                   Capsid Mutation(s) AAV2                                    Y730F AAV2                                    Y444F AAV2                                    Y444F+Y730F AAV2                                    Y444F+Y730F+Y500F AAV2                                    Y444F+Y730F+Y500F+Y272F AAV8                                    Y733F AAV8                                    Y447F AAV9                                    Y446F AAV9                                    Y731F AAV9                                    Y446F + Y731F

What is WPRE?

Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of […]