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Converting dsDNA from Nanograms to Copy Number

Researchers performing qPCR will often create a standard curve based on nanograms of amplicon, and then need to convert the resulting nanograms detected to copy number. The formula for making this conversion is as follows:

Where: X = amount of amplicon (ng)
N = length of dsDNA amplicon
660 g/mole = average mass of 1 bp dsDNA

† The actual oligonucleotide MW (in Daltons or g/mole) is provided on the IDT Spec Sheet for each oligonucleotide and can be substituted for this arithmetic phrase, which only provides an average MW for a sequence of this length.

An alternative is to create the standard curve based on copy number; e.g., ranging from 102 to 107 copies, in 10-fold increments. You can then determine copy number directly from the standard curve.

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