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Cre-inducible Adeno-associated Virus: DIO-AAV

For cell type-specific targeting and to capitalize on the large number of Cre driver lines with the flexible virus injection/fiberoptic approach, we have developed a tool we call DIO-AAV (doublefloxed […]

Primers for Lentivirus Titration using qRT-PCR

Vector and region amplified Primers and probes dedicated for the amplification of lentiviral or transgenic DNA sequences Primers and probes dedicated for the amplification of internal control DNAs (cellular genome […]

Home-made Gateway Clonase II

1.Excision reaction. 44.5 mM Tris–borate (pH 8.3) 7.5 mM Tris–HCl (pH 8.0) 1.15 mM EDTA 5 mM spermidine 60 mM KCl 0.275 mg/ml BSA 15% (v/v) glycerol. Int 50~100 nM […]

SignaGen’s In Vitro siRNA Transfection Reagents

siRNA Transfection Reagent Selection Chart. Reagents Features Transfection Type Toxicity PepMute™ – Exceptional good for siRNA transfection – OK for DNA/siRNA co-transfection siRNA (Yes) DNA/siRNA (No) ++ GenMute™ – Exceptional […]

>300 Pre-made Ready to Use Adenoviruses from SignaGen

SiganGen is offering >300 pre-made and ready to use adenovirus vectors.  All the pre-packaged adenoviruses were packaged from Ad.MAX™ system with a serotype 5 adenoviral backbone* (E1/E3 deletion).  All the […]

SignaGen’s rAAV Production Service

Multi Approaches to Produce Super Infectious rAAV Particles at Super High Titer: Not like adenovirus, it is relatively harder to achieve high yield of rAAV with regular rAAV production procedures. […]