Miscellaneous - 3. page

Product Name Gram (+)bacteria Gram (-)bacteria Yeasts Molds Mycoplasma Suggested Working Conc. Amphotericin B     † †   2.5 mg/L Amphotericin B-Solubilized (Approx. 45%)     † †   […]

Plasmid purification is a common technique in most molecular biology labs. While the standard alkaline lysis method had been well established, a surprising number of things still can go wrong. […]

5′-TTTGCAAATTCATAAGTAGTCTGCC-3′5′-CGTCACTGCGCATGCGTAT-3′

5′- CCGTTGGTGTATCTTTGAATA -3′

5′-GGACTTAAAGGCTCTTAAT-3′5′-GGACAAGAAAGCAGACAAA-3′

CCTCAGAGAGATCAGAGAGTT

5′- GACAGCATCGTGTCTGTAG -3′5′- ATCGTGTCTGTAGGACAGC -3′

5′- GGAAAGCTCTATACCTGTT – 3′

In Stage 1, Set to 2 min at 25°C; 1 Rep.In Stage 2, Set to 15 min at 55°C; 1 Rep.In Stage 3, Set to 2 min at 95°C, 1 […]

5′-GGGTGAAGATCCTGTTCAATA-3′