Ad-mCherry-PA-Rac1-T17N

Price: $688.00

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  • Catalog #: SL101112
  • Unit Size: 1 x 200 µL

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Product Category: human Adenovirus Type5 (dE1/E3), mCherry-PA-Rac1-T17N adenovirus for over-expression of mCherry-PA-Rac1 mutant (T17N) under CMV promoter; Ad-CMV-mCherry-PA-Rac1-T17N; Ad-mCherry-PA-Rac1-T17N

Description:
Rac1 is a key GTPase regulating actin cytoskeletal dynamics in metazoan cells. Rac1 mutants were fused to the photoreactive LOV (light oxygen voltage) domain from phototropin, sterically blocking Rac1 interactions until irradiation unwound a helix linking LOV to Rac1. Photoactivatable Rac1 (PA-Rac1) could be reversibly and repeatedly activated using 458- or 473-nm light to generate precisely localized cell protrusions and ruffling. A mutation at T17N abolished this effect.  Ad-CMV-mCherry-PA-Rac1-T17N will over-express mCherry-PA-Rac1 (T17N) in most of mammalian cell types in which Rac activation won't happen thru photoactivation, serving as a control for Ad-CMV-mCherry-PA-Rac1. 

Titer:
1x1010 ~ 1x1011 PFU/mL

Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol

User Manual:


Shuttle Vector Map:
Ad-CMV-mCherry-PA-Rac1-T17N; Ad-mCherry-PA-Rac1-T17N; Pre-made Adenovirus

 




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