LV-CMV-GCaMP6f-T2A-mScarlet

Product Category: Pre-made recombinant lentivirus; LV-CMV-GCaMP6f-T2A-mScarlet; CMV-GCaMP6f Lentivirus

Description: 
GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase.  When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms.

GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level.  Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast - GCamP6f, slow - GCaMP6f and medium - GCaMP6m). 

LV-CMV-GCaMP6f-T2A-mScarlet is a pre-made lentivirus which expresses GCaMP6f under the CMV promoter with co-expression of Scarlet as reporter via T2A linker. Ready to use format.

Titer: > 1x10⁹ TU/mL

Selection Antibiotic: None

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol: 

Transfer Vector Map:


* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm