Pre-made Adenovirus,Ad-CMV-Gluc; Ad-CMV-hGluc from SignaGen
Product Category: Human Adenovirus Type5 (dE1/E3); Ad-CMV-Gluc; Ad-CMV-hGluc
Description:
This recombinant human adenovirus type 5 over-expresses codon-optimized/humanized
Gaussia luciferase under CMV promoter. The humanized form of Gaussia luciferase
(hGLuc) was confirmed to be nontoxic and naturally secreted after
over-expression in mammalian cells. hGLuc generated over 1000-fold higher
bioluminescent signal intensity from live cell and over 100-fold higher
intensity from viable cells alone (not including secreted luciferase) or cell
lysates, compared to humanized forms of firefly (hFLuc) and Renilla (hRLuc)
luciferases expressed under similar conditions. Furthermore, hGLuc showed
200-fold higher signal intensity than hRLuc and intensity comparable to that of
hFLuc in vivo under standard imaging conditions. Gaussia luciferase provides a
sensitive means of imaging gene delivery and other events in living cells in
both tissue culture and in vivo, with a unique combination of features including
high signal intensity, secretion, and ATP independence, thus being able to
report from the cells and their environment in real time. Ready to use format.
Titer: 1x1010 ~ 1x1011 PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
User Manual:
Shuttle Vector Map:
