AAV-CAG-DIO-ChIEF-tdTOMATO (AAV Serotype 1)

Price: $488.00

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  • Catalog #: SL100900
  • Unit Size: 1 x >30 µL (>1E+13 VG/mL)

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Product Category: Pre-made recombinant AAV; AAV-CAG-DIO-ChIEF-tdTOMATO; AAV1-CAG-DIO-ChIEF-tdTOMATO

Description:

AAV1-CAG-DIO-ChIEF-tdTOMATO is a pre-made rAAV in serotype 1 which will Cre dependently over-express DIO-ChIEF-tdTOMATO driven under CAG promoter (also known as CBA promoter) as illustrated as the cartoon below.

ChIEF is a hybrid of channelrhodopsin 1 and 2 with additional L170I mutation (ChR1 numbering) or at L131I (ChR2-numbering), leading to large currents in oocytes and HEK-cells and almost wild type like open state life time.
ChIEF function as light-gated ion channels and very useful for many bioengineering and neuroscience applications such as photostimulation of neurons for probing of neural circuits. Using tdTOMATO tagged ChIEF, light-stimulated axons and synapses can be identified in intact brain tissue. This is useful to study the molecular events during the induction of synaptic plasticity. ChIEF has also been used to map long-range connections from one side of the brain to the other, and to map the spatial location of specific inputs on the dendritic tree of individual neurons.

In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5' promoter and is flanked by oppositely oriented loxP and lox2272 sites.  In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be "Flip-exchanged" or FLEXed, leading to the expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV). 

AAV-CAG-DIO-ChiEF-tdTOMATO


Titer: > 1x1013 VG/mL

Size: > 30 µL (Std Pack)

Storage Buffer: Sterile PBS

Infection Protocol:


Vector Map:
AAV-CAG-DIO-ChIEF-tdTOMATO; AAV1-CAG-DIO-ChIEF-tdTOMATO



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