GenJet™ In Vitro DNA
Tranfection Reagent (Ver. II) is upgraded version of GenJet™ In Vitro DNA
Transfection Reagent. Adopting a new chemistry, more DNA condensing groups were
liberated in GenJet™ (Ver. II) (100 % vs. 80% in old version), leading to 3~4
folds increase of transfection efficiency without sacrificing cell viability.
GenJet™ (Ver. II) reagent, 1.0 ml, is sufficient for ~667 transfections in
24 well plates or ~333 transfections in 6 well plates.
compared with old version GenJet™ In Vitro DNA Trasnfection Reagent
- Cell-dependent 3~4 times
- Top choice for hard-to-transfect cells
- Efficiency boosted in the presence of serum and antibiotics for most of cell
- Exceptional high levels of recombinant protein production
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Comparisons of Transfection Efficiency and Cytotoxicity of GenJet™ (Ver. II)
with Old Version GenJet™ and Other Leading Products
Comparison of transfection efficiency (left
panel) and toxicity (right panel) GenJet™ Ver. II with old version GenJet™ on
commonly used mammalian cells. phRL-CMV encoding Renilla Luciferase was
introduced into cells with the presence of serum and antibiotics. The Renillia
Luciferase activity was determined with Renilla Luciferaase Assay System (Promega).
For cytotoxicity, MTT assay was employed to determine cell viability with
untransfected control as 100%.
A comparison of transfection efficiency of
GenJet™ (Ver. II) vs. Lipofectamine 2000 on primary rat aortic smooth muscle cells.
Primary rat aortic smooth muscle cells were prepared and transfected with a
nuclear targeted GFP cDNA by GenJet™ (right
panel) and Lipofectamine 2000 (L2K, left panel) respectively. The
transfection efficiency were checked under a Nikon Eclipse 2000 fluorescence
microscopy 48 hours post transfection
Upper Panel: Transfection efficiency
comparison of GenJet™ Ver. II with old version GenJet™ on 293T cells. pEGFP-N3
was transfected to 293T cells at 90% confluency with presence of serum and
antibiotics. GFP fluorescence was detected with Nikon Eclipse 2000 microscopy 48
hours post transfection
Lower panel: Transfection efficiency comparison of GenJet™ Ver. II with
old version GenJet™ on Hela cells. pEGFP-N3 was transfected to Hela cells at 90%
confluency with presence of serum and antibiotics. GFP fluorescence was detected
with Nikon Eclipse 2000 microscopy 48 hours post transfection
Information & Datasheet
Short Protocol for Advanced Users
Protocol for Lentivirus Production
Protocol rAAV Production
GenJet™ In Vitro DNA Transfection reagent
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