Description:
GenMute™
siRNA Transfection Reagent for Jurkat Cell is pre-optimized for transfecting
siRNA to Jurkat cells. Jurkat
cells are an immortalized line of T lymphocyte cells that are used to
study acute T cell leukemia, T cell signaling, and the expression of various
chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells
are also useful in science because of their ability to produce interleukin 2.
Their primary use, however, is to determine the mechanism of differential
susceptibility of cancers to drugs and radiation.
Size & content:
-
GenMute™ Reagent,
1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles
siRNA or miRNA mimics in 24-well plate
-
GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml
to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on
Jurkat cells.
GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
|
Summary of
Optimal Transfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA
Complex
Maximal Silencing Efficiency
Transfection
Results:
Reporter Gene
Silencing
Efficiency (% ) |
1640 Medium with 10% FBS
~50%
40 nM
1xGenMute™
Transfection Buffer
~15 minutes at RT
48 hours
Renilla Luciferase
80% |
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
