Description:
GenMute™
siRNA Transfection Reagent for MCF7 Cell is pre-optimized for transfecting siRNA
to MCF7 cells. MCF-7 cell is a
breast cancer cell line was isolated in 1970 from a 69-year-old Caucasian woman.
This cell line retained several characteristics of differentiated mammary
epithelium including the ability to process estradiol via cytoplasmic estrogen receptors and the capability of
forming domes. This cell line expresses the oncogene Wnt7B. Tumor necrosis
factor alpha (TNF alpha) inhibits the growth of MCF-7 breast cancer cells.
Treatment with anti-estrogens can modulate the secretion of insulin-like growth
factor binding protein's.
Size & content:
-
GenMute™ Reagent,
1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles
siRNA or miRNA mimics in 24-well plate
-
GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml
to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on
MCF7 cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Cell culture
medium
Confluency
of cell on the
day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA
Complex
Maximal Silencing Efficiency
Transfection
Results:
Reporter Gene
Silencing
Efficiency (% ) |
DMEM with 4.5 g/L glucose, 10% FBS
~50%
40 nM
1xGenMute™
Transfection Buffer
~15 minutes at RT
48 hours
Renilla Luciferase
90% |
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
