Description
GenJet™ DNA In Vitro
Transfection Reagent for
B16-F10 cell is pre-optimized for transfecting
B16-F10 cells. B16-F10 is a derived murine melanoma cell line, which is
well-established model for the study of experimental cancer therapies.
Melanomas express different tumor-associated antigens, which are potential
targets for novel designs of therapeutic cancer vaccines.
Melanoma-associated antigens include tyrosinase and tyrosine-related protein (TRP)-1/2
or the MAGE and BAGE class of antigens. Many immunotherapeutic protocols
have been tested using the murine B16-F10 melanoma cell line that originated in
the syngeneic C57BL/6 (H-2b) mouse strain.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on B16-F10 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~90%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours
EGFP
pEGFP-N3 (CMV promoter)
88% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
