Description
GenJet™ DNA In Vitro
Transfection Reagent for C6 Glioma cell is pre-optimized for transfecting C6
glioma cells.
The C6 glioma cell line is a widely used cell line in neurobiological research.
Compared to primary neural cells, the C6 cell line offers some major advantages
like easy accessibility and culture as well as a good availability of high cell
numbers. Nevertheless, the biggest disadvantage of the C6 cells is that
they are difficult-to-transfect with commonly used non-viral transfection
methods. The pre-optimized transfection reagent simplifies the transfection of
C6 cells. Transfection efficiencies of over 60% can be easily achieved with
excellent cell viabilities of above 90%.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on C6 glioma cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
~80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours
EGFP
pEGFP-N3 (CMV promoter)
60% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
