Description
GenJet™ DNA In Vitro
Transfection Reagent for 3LL cell is pre-optimized for transfecting 3LL cells.
3LL also called
Lewis lung
carcinoma was discovered by Dr. Margaret R. Lewis of the Wistar Institute in
1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL
mouse. The tumor does not appear to be grossly hemorrhagic and the majority of
the tumor tissue is a semifirm homogeneous mass.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on 3LL cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
|
Summary of
Optimal Transfection Conditions:
Confluence on the
day of transfection
Cell culture
conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA
Complex
Presence of Serum/Antibiotics
during Transfection
Change Medium After Transfection
Maximal Efficiency
Transfection
Results:
Reporter Gene
Plasmid
Efficiency (GFP %) |
80%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, 18~24 Hours After Transfection
48 hours
EGFP
pEGFP-N3 (CMV promoter)
88% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on 3LL Cells. 3LL cells
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
