CompLysis™ Protein Extraction Reagen

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Application: Mamallian Cell Protein Extraction for Western Blot and Other Protein Extraction Applications

FEATURES:
- Non-ionic detergent formulation, suitable for Bradford and BCA Protein Assays
- Keep the extracted protein undenatured, suitable for any affinity purification procedures like immunoprecipitation
- Keep GST, luciferase, beta-galactosidase, CAT and other reporter/tag gene activities

Comparison of CompLysis™ Stripping Reagent with the freeze/thaw cycle, sonication and the Most Popular Brand Product M:

* HEK293 cells grown in 10 cm plates at full confluency. CompLysis™ Reagent is utilized to extract total protein. For ß-galactosidase assay, HEK293 cells were transfected with reporter construct expressing ß-galactosidase.

Data Sheet