Western Blotting Products

Protein blotting is an analytical method that involves the immobilization of proteins on membranes before detection using monoclonal or polyclonal antibodies. There are different blotting protocols (dot blot, 2D blot); one of the most powerful is western blotting.
Western Blotting or immunoblotting allows investigators to determine, with a specific primary antibody, the relative amounts of the protein present in different samples. Briefly, 1) Samples are prepared from tissues or cells that are homogenized in a buffer that protects the protein of interest from degradation; 2) The sample is separated using SDS-PAGE and then transferred to a membrane for detection; 3) The membrane is incubated with a generic protein (such as milk proteins) to bind to any remaining sticky places on the membrane. A primary antibody is then added to the solution which is able to bind to its specific protein; 4) A secondary antibody-enzyme conjugate, which recognizes the primary antibody is added to find locations where the primary antibody bound.