Miscellaneous - 3. page

Plasmid purification is a common technique in most molecular biology labs. While the standard alkaline lysis method had been well established, a surprising number of things still can go wrong. […]

5′-TTTGCAAATTCATAAGTAGTCTGCC-3′5′-CGTCACTGCGCATGCGTAT-3′

5′- CCGTTGGTGTATCTTTGAATA -3′

5′-GGACTTAAAGGCTCTTAAT-3′5′-GGACAAGAAAGCAGACAAA-3′

CCTCAGAGAGATCAGAGAGTT

5′- GACAGCATCGTGTCTGTAG -3′5′- ATCGTGTCTGTAGGACAGC -3′

5′- GGAAAGCTCTATACCTGTT – 3′

In Stage 1, Set to 2 min at 25°C; 1 Rep.In Stage 2, Set to 15 min at 55°C; 1 Rep.In Stage 3, Set to 2 min at 95°C, 1 […]

5′-GGGTGAAGATCCTGTTCAATA-3′

Name Description Oligonucleotide Sequence (5’>3’) Label1 FinalConc. 2019-nCoV_N1-F 2019-nCoV_N1 Forward Primer GAC CCC AAA ATC AGC GAA AT None 500nM 2019-nCoV_N1-R 2019-nCoV_N1 Reverse Primer TCT GGT TAC TGC CAG TTG […]