There is DNA contamination somewhere in your PCR assay system. Use only PCR-grade reagents and lab ware. Wear gloves throughout the procedure. Always use fresh pipette tips, water and other […]
Miscellaneous - 4. page
qPCR: Avoiding Signals in the No-template Control (NTC)
This month’s question from the Molecular Biology Forums (online at molecularbiology.forums.biotechniques.com) comes from the “Real-time qPCR/qRT-PCR Methods” section. Entries have been edited for concision and clarity. Mentions of specific products […]
Human HEY1 siRNA Validated
5′-UAGAGCCGAACUCAAGUUUCCAUUC-3′ 5′-UUGAGAUGCGAAACCAGUCGAACUC-3′
EGFP Primers and Probe for qPCR
Primers: 5’-AGTCCGCCCTGAGCAAAGA-3’ (eGFP-F) 5’-TCCAGCAGGACCATGTGATC-3’ (eGFP-R) Probe: CCCAACGAGAAGCG
Validated Target Region against Mouse Lamtor1
CGTATGCCTATAGTGCACTTT
Two Validated Target Regions against Mouse ROCK2
CAGAAGCGTTGTCTTATGCAA TTGGATAAACATGGACATCTA
Two validated target regions
N-Taf : CTCAGGCTAAGCCTCCTGATT C/N-Taf: TGGAGGAAGCAGAATTAGAAA
Validated Target Region against Mouse Rock2
5’-CAGAAGCGTTGTCTTATGCAA-3’ 5’-TTGGATAAACATGGACATCTA-3’
Human VMD2 Promoter
AATTC TGTCATTTTA CTAGGGTGAT GAAATTCCCA AGCAACACCATCCTTTTCAG ATAAGG GCAC TGAGGCTGAG AGAGGAGCTG AAACCTACCC GGCGTCACCACACACAGGTG GCAAG GCTGG GACCAGAAAC CAGGACTGTT GACTGCAGCC CGGTATTCATTCTTTCCATA GCCCA CAGGG CTGTCAAAGA CCCCAGGGCC TAGTCAGAGG CTCCTCCTTCCTGGAGAGTT CCTGG CACAG AAGTTGAAGC TCAGCACAGC CCCCTAACCC CCAACTCTCTCTGCAAGGCC TCAGG […]
Validated Target Region against Mouse TIMP1 Gene
# 1. tgcacagtgtttccctgtt # 2. tcaagatgactaagatgct # 3. tgcacagtgtttccctgtt