Summary In 2009 Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear DNA fragments (Nat Methods 2009;6(5):343-5). […]
Miscellaneous - 2. page
Tips for Maximizing Ligation Efficiencies
T4 DNA Ligase is the most extensively used ligase for cloning-based experiments. Traditionally, a ligation reaction (blunt or cohesive ends) using traditional T4 DNA Ligase involves incubation at 16°C using […]
Troubles with ligation?
Check the activity of your T4 DNA Ligase with two easy-to-do control experiments You may check the efficiency of your ligation reaction by mixing the reaction with loading dye containing […]
Electroporation vs. Heat Shock Transformation
The advantages of using electroporation are the higher efficiency, more colonies, and much faster transformations compared to heat shock method. Transformation efficiencies for electroporation are 5.0 x 109 – 2.0 x […]
DNA/RNA non-specific endonuclease [Serratia marcescens]
atgcgctttaacaacaagatgttggccctggccgccctgctgttcgccgcacaggcgtcggccgacacgttcgaatccatcgacaactgcgcggtcggctgcccgaccggcggcagcagcaacgtgtcgatcgtgcgtcatgcttatacgttgaacaacaacagcacaaccaagttcgccaactgggtggcctatcacatcaccaaagacacgccggccagcggcaagacgcgcaactggaaaaccgacccggcgctcaatccggccgacaccctggcgcccgccgattacaccggcgccaacgcggcgctgaaggtcgatcgcggtcatcaggcgccgctggcctcgctggcgggcgtttccgactgggaatcgctgaactacctgtccaacatcacgccgcaaaagtccgatcttaaccagggcgcctgggcgcggctggaagatcaggaacgcaagctgatcgatcgcgccgacatctcctcggtctataccgtgaccgggccgttgtatgagcgtgatatgggcaaactgccgggcacccagaaagcgcacaccatccccagcgcctactggaaggtgattttcatcaacaacagcccggcggtaaaccactatgccgctttcctgttcgatcagaacacgccgaagggcgccgatttctgccaattccgcgtgacggtggacgagatcgagaaacgtaccggcctgatcatctgggccggtctgccggacgacgtacaggcttcgctgaagagcaagcccggcgtcctgccggagctgatgggctgcaaaaactga
DNA/RNA non-specific endonuclease [Serratia marcescens]
ATGCGCTTCTCCAACAAAATGCTTGCGTTGGCAGCACTGCTGTTTGCAGCGCAAGCGAGTGCAGATACCCTGGAGTCGATTGACAATTGTGCTGTGGGTTGTCCGACAGGAGGCAGCAGTAACGTGAGCATCGTACGTCATGCGTATACCCTGAACAACAATAGCACCACCAAATTTGCGAATTGGGTGGCGTACCATATTACGAAGGACACGCCGGCCTCTGGGAAAACCCGGAATTGGAAAACCGATCCCGCGCTGAATCCGGCCGATACTCTGGCACCAGCCGACTATACAGGCGCGAATGCTGCTCTGAAAGTGGATCGTGGGCATCAAGCCCCTTTAGCGTCACTTGCCGGCGTTTCTGACTGGGAATCGCTCAACTATCTCAGCAACATTACGCCGCAGAAATCTGACCTTAACCAGGGTGCATGGGCACGCTTGGAAGATCAGGAACGCAAGCTGATTGATCGTGCGGACATTTCATCCGTCTACACCGTTACTGGTCCGCTGTATGAACGCGATATGGGCAAATTACCGGGCACGCAGAAAGCGCACACGATTCCCAGTGCTTACTGGAAAGTGATCTTCATCAACAATTCGCCTGCGGTCAACCACTATGCTGCGTTCCTGTTCGATCAGAACACGCCAAAAGGTGCCGACTTTTGCCAGTTTCGCGTAACCGTAGATGAAATCGAGAAACGTACCGGCCTGATCATCTGGGCCGGTTTACCGGATGATGTGCAGGCATCCTTGAAAAGCAAACCGGGCGTTCTGCCAGAACTGATGGGTTGCAAGAATTAA
DNA/RNA non-specific endonuclease [Serratia marcescens]
1 MRFNNKMLAL AALLFAAQAS ADTLESIDNC AVGCPTGGSS NVSIVRHAYT LNNNSTTKFA 61 NWVAYHITKD TPASGKTRNW KTDPALNPAD TLAPADYTGA NAALKVDRGH QAPLASLAGV 121 SDWESLNYLS NITPQKSDLN QGAWARLEDQ ERKLIDRADI SSVYTVTGPL YERDMGKLPG 181 TQKAHTIPSA YWKVIFINNS PAVNHYAAFL FDQNTPKGAD FCQFRVTVDE IEKRTGLIIW 241 AGLPDDIQAS […]
Density and Refractive Index for CsCl
Density (g/cm^3) Refractive index 1.0047 1.3333 1.0125 1.334 1.0204 1.3348 1.0284 1.3356 1.0365 1.3364 1.0447 1.3372 1.0531 1.338 1.0615 1.3388 1.07 1.3397 1.0788 1.3405 1.0877 1.3414 1.0967 1.3423 1.1059 1.3432 […]
Cell Culture Antibiotic Selection Guide
Product Name Gram (+)bacteria Gram (-)bacteria Yeasts Molds Mycoplasma Suggested Working Conc. Amphotericin B † † 2.5 mg/L Amphotericin B-Solubilized (Approx. 45%) † † […]