Miscellaneous - 2. page

Conclusion In conclusion, the absence of apparent supercoiling in plasmid DNA preparations can result from a variety of factors related to DNA extraction, purification, handling, and analysis. By systematically addressing […]

Successful transient transfection relies on many factors, starting with a successful plasmid prep. To ensure high transfection efficiency, resulting DNA from your plasmid preps must be transfection grade. But what […]

When uncut plasmid DNA is isolated and run on an agarose gel, you may observe two, three, or even four or more bands. Hopefully, the majority of your isolated DNA […]

For a 20µl reaction: Component   Volume (µl)   Final Concentration H2O 12.9   10x homemade core buffer 2 1x 2mM dNTP mix 2 0.2mM 20µM primer mix 0.5 500nM […]

Reagent Volume (for 150 µL) qPCR mix (2×) 100 µL Sense primer (1 µM) 2 µL Antisense primer (1 µM) 2 µL Fluorescein (1 µM) 2 µL SYBR Green (40×) […]

As DNA is a highly hydrophilic molecule, it usually cannot pass through the cell membrane of bacteria. Hence, to make bacteria capable of internalizing genetic material, they must be made […]

A common use for orbital shakers is to grow bacteria for a variety of purposes, including the production of proteins and genetic matter and for the study of bacteria themselves. […]

Summary In 2009 Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear DNA fragments (Nat Methods 2009;6(5):343-5). […]

T4 DNA Ligase is the most extensively used ligase for cloning-based experiments. Traditionally, a ligation reaction (blunt or cohesive ends) using traditional T4 DNA Ligase involves incubation at 16°C using […]

Check the activity of your T4 DNA Ligase with two easy-to-do control experiments You may check the efficiency of your ligation reaction by mixing the reaction with loading dye containing […]