5′- GGAAAGCTCTATACCTGTT – 3′
Miscellaneous - 3. page
RT-qPCR Reaction Conditions for Detection of Covid-19 by FDA and CDC
In Stage 1, Set to 2 min at 25°C; 1 Rep.In Stage 2, Set to 15 min at 55°C; 1 Rep.In Stage 3, Set to 2 min at 95°C, 1 […]
Validated Target Region against Mouse PTBP1 Gene
5′-GGGTGAAGATCCTGTTCAATA-3′
Research Use Only 2019-Novel Coronavirus (2019-nCoV, Covid-19) Real-time RT-PCR Primers and Probes
Name Description Oligonucleotide Sequence (5’>3’) Label1 FinalConc. 2019-nCoV_N1-F 2019-nCoV_N1 Forward Primer GAC CCC AAA ATC AGC GAA AT None 500nM 2019-nCoV_N1-R 2019-nCoV_N1 Reverse Primer TCT GGT TAC TGC CAG TTG […]
Bmi-1 Validated target Regions
GAGATAATAAGCTTGTCTA ACAACCAGAATCAAGATCA ATATGGATGTTAAGTGGAA
Validated siRNA Targeting Mouse Trpv1 Gene
5’-GTCGGTTTATGTTCGTCTAC-3’
Why do I see amplification curves in my NTC samples?
Products can appear in non-template control (NTC) wells due to the ability of some primer sequences to form structures that can be used as substrates by DNA polymerases. The most common […]
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
There is DNA contamination somewhere in your PCR assay system. Use only PCR-grade reagents and lab ware. Wear gloves throughout the procedure. Always use fresh pipette tips, water and other […]
qPCR: Avoiding Signals in the No-template Control (NTC)
This month’s question from the Molecular Biology Forums (online at molecularbiology.forums.biotechniques.com) comes from the “Real-time qPCR/qRT-PCR Methods” section. Entries have been edited for concision and clarity. Mentions of specific products […]
Human HEY1 siRNA Validated
5′-UAGAGCCGAACUCAAGUUUCCAUUC-3′ 5′-UUGAGAUGCGAAACCAGUCGAACUC-3′