1 cagagaggga gtggccaact ccatcactag gggtagcgcg aagcgcctcc cacgctgccg 61 cgtcagcgct gacgtaaatt acgtcatagg ggagtggtcc tgtattagct gtcacgtgag 121 tgcttttgcg gcattttgcg acaccacgtg gccatttgag gtatatatgg ccgagtgagc 181 gagcaggatc tccattttga ccgcgaaatt tgaacgagca gcagccatgc cgggcttcta 241 cgagatcgtg […]
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AAV1 Complete Genome
1 ttgcccactc cctctctgcg cgctcgctcg ctcggtgggg cctgcggacc aaaggtccgc 61 agacggcaga gctctgctct gccggcccca ccgagcgagc gagcgcgcag agagggagtg 121 ggcaactcca tcactagggg taatcgcgaa gcgcctccca cgctgccgcg tcagcgctga 181 cgtaaattac gtcatagggg agtggtcctg tattagctgt cacgtgagtg cttttgcgac 241 attttgcgac […]
AAV5 Complete Genome
1 ctctcccccc tgtcgcgttc gctcgctcgc tggctcgttt gggggggtgg cagctcaaag 61 agctgccaga cgacggccct ctggccgtcg cccccccaaa cgagccagcg agcgagcgaa 121 cgcgacaggg gggagagtgc cacactctca agcaaggggg ttttgtaagc agtgatgtca 181 taatgatgta atgcttattg tcacgcgata gttaatgatt aacagtcatg tgatgtgttt 241 tatccaatag […]
AAV2 Complete Genome
1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 61 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg 121 gccaactcca tcactagggg ttcctggagg ggtggagtcg tgacgtgaat tacgtcatag 181 ggttagggag gtcctgtatt agaggtcacg tgagtgtttt gcgacatttt gcgacaccat 241 gtggtcacgc […]
AAV2 Capsid Protein VP3
10 20 30 40 50 MATGSGAPMA DNNEGADGVG NSSGNWHCDS TWMGDRVITT STRTWALPTY 60 70 80 90 100 NNHLYKQISS QSGASNDNHY FGYSTPWGYF DFNRFHCHFS PRDWQRLINN 110 120 130 140 150 NWGFRPKRLN FKLFNIQVKE VTQNDGTTTI ANNLTSTVQV FTDSEYQLPY […]
AAV2 Capsid Protein VP2
10 20 30 40 50 MAPGKKRPVE HSPVEPDSSS GTGKAGQQPA RKRLNFGQTG DADSVPDPQP 60 70 80 90 100 LGQPPAAPSG LGTNTMATGS GAPMADNNEG ADGVGNSSGN WHCDSTWMGD 110 120 130 140 150 RVITTSTRTW ALPTYNNHLY KQISSQSGAS NDNHYFGYST PWGYFDFNRF […]
AAV2 Capsid Protein VP1
10 20 30 40 50 MAADGYLPDW LEDTLSEGIR QWWKLKPGPP PPKPAERHKD DSRGLVLPGY 60 70 80 90 100 KYLGPFNGLD KGEPVNEADA AALEHDKAYD RQLDSGDNPY LKYNHADAEF 110 120 130 140 150 QERLKEDTSF GGNLGRAVFQ AKKRVLEPLG LVEEPVKTAP GKKRPVEHSP […]
AAV 2 vector: Point mutations in tyrosines lead to high-efficiency transduction.
AAV2-mediated transduction of hepatocytes from normal C57BL/6 mice injected via tail vein with tyrosine-mutant capsid scAAV2-EGFP vectors. (A) Transgene expression was detected by fluorescence microscopy 2 weeks after injection of […]
The WPRE reduces readthrough transcription from retroviral vectors
The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed […]
What is WPRE?
Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of […]