MAADGYLPDWLEDNLSEGIREWWDLKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEPSPQRSPDSSTGIGKTGQQPAKKRLNFGQTGDSESVPDPQPIGEPPAGPSGLGSGTMAAGGGAPMADNNEGADGVGSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGSTNDNTYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNEGTKTIANNLTSTIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFEFSYQFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQSTGGTAGTQQLLFSQAGPNNMSAQAKNWLPGPCYRQQRVSTTTGQNNNSNFAWTAGTKYHLNGRNSLANPGIAMATHKDDEERFFPSNGILIFGKQNAARDNADYSDVMLTSEEEIKTTNPVATEEYGIVADNLQQQNTAPQIGTVNSQGALPGMVWQNRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGLKHPPPQILIKNTPVPADPPTTFNQSKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSTSVDFAVNTEGVYSEPRPIGTRYLTRNL
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Homemade qPCR Mix Recipe
For a 20µl reaction: Component Volume (µl) Final Concentration H2O 12.9 10x homemade core buffer 2 1x 2mM dNTP mix 2 0.2mM 20µM primer mix 0.5 500nM […]
Home made qPCR Master Mix
Reagent Volume (for 150 µL) qPCR mix (2×) 100 µL Sense primer (1 µM) 2 µL Antisense primer (1 µM) 2 µL Fluorescein (1 µM) 2 µL SYBR Green (40×) […]
Nanopore Sequencing: Principles, Platforms and Advantages
History of DNA sequencing Over the last fifty years, a number of researchers have devoted themselves to the development of DNA sequencing technology. This time-scale has witnessed drastic changes, moving […]
Nanopore Sequencing
Nanopore sequencing is a third generation approach used in the sequencing of biopolymers — specifically, polynucleotides in the form of DNA or RNA. Using nanopore sequencing, a single molecule of […]
The Principle of Transformation
As DNA is a highly hydrophilic molecule, it usually cannot pass through the cell membrane of bacteria. Hence, to make bacteria capable of internalizing genetic material, they must be made […]
IRES or 2A in my polycistronic expression cassette, which one is better?
When designing a gene expression vector to co-express multiple ORFs under the control of a single promoter, you can choose to place multiple ORFs behind the promoter, separated by linkers […]