1. ITR Structure and Function• ITRs are crucial: ITRs are key elements in AAV vectors, serving as origins of replication, and are essential for viral genome replication, packaging into capsids, […]
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How Does pH Affect DNA Stability?
In a neutral pH range, pH 5 to 9, DNA molecules are quite stable. However, if the pH becomes too acidic or alkaline, DNA molecules are prone to destabilization. At […]
What is pH Value of ddH2O?
The pH of double-distilled water (ddH₂O) is theoretically around 7.0 — perfectly neutral — because pure water at 25°C autoionizes slightly into H₃O⁺ and OH⁻ ions. But in practice, ddH₂O […]
How many adenovirus particles can be made in one HEK293 cell?
HEK293 cells can produce over 100,000 adenovirus particles per cell when using optimized protocols and transfection methods. Here’s a more detailed explanation: