| Applicability | Target | Sequences |
| Universal All rAAV samples | ITR2 | primers: 5′-ggaacccctagtgatggagtt-3′ and 5′-cggcctcagtgagcga-3′; probe: R-5′-cactccctctctgcgcgctcg-3′-Q. |
| Semi-universal Group of rAAV samples | CMV promoter | primers: 5′-ttcctacttggcagtacatctacg-3′ and 5′-gtcaatggggtggagacttgg-3′; probe: R-5′-tgagtcaaaccgctatccacgccca-3′-Q; and other sequences. |
| CAG promoter | primers: 5′-ctgaccgcgttaatcccaca-3′ and 5′-acaagccgtgattaaaccaaga-3′. | |
| CBA promoter | primers: 5′-ccgcagccattgccttt-3′ and 5′-ccgcacagatttgggacaa-3′; probe: R-5′-atggtaatcgtgcgagagggcgc-3′-Q. | |
| TBG promoter | primers: 5′-aaactgccaattccactgctg-3′ and 5′-ccataggcaaaagcaccaaga-3′; probe: R-5′-ttggcccaatagtgagaactttttcctgc-3′-Q. | |
| GRK1 promoter | primers: 5′-tctcttaaggtagccccgg-3′ and 5′-atccgattagatcattctgccc-3′; probe: R-5′-cctcacttttcccctgagaaggaca-3′-Q. | |
| RBG intron | primers: 5′-tcaggtgcaggctgcctat-3′ and 5′-tttgtgagccagggcattg-3′; probe: R-5′-agaaggtggtggctggtgtgg-3′-Q. | |
| CMV enhancer | primers: 5′-gtcaatgggtggagtatttacgg-3′ and 5′-gcattatgcccagtacatgacct-3′; probe: R-5′-caagtgtatcatatgccaagtacgccccc-3′-Q; and other sequences. | |
| WPRE | primers: 5′-ttggatgctcgcctgggttg-3′ and 5′-aggaaggtccgctggatcga-3′. | |
| SV40 polyA | primers: 5′-agcaatagcatcacaaatttcacaa-3′ and 5′-ccagacatgataagatacattgatgagtt-3′; probe: R-5′-agcatttttttcactgcattctagttgtggtttgtc-3′-Q. | |
| BGH polyA | primers: 5′-catataaaatgaggaaattgcatcgca-3′ and 5′-tcagaacccatagagcccaccg-3′; and other sequences. | |
| RBG polyA | primers: 5′-gatttttcctcctctcctgactactc-3′ and 5′-gctgcaggtcgagggatct-3′; probe: R-5′-cagtcatagctgtccctcttctctt-3′-Q; and other sequences. | |
| Individual Unique rAAV samples | eGFP | primers: 5′-cacccacgtgaccacccttac-3′ and 5′-ggatgttgcagtcctccctg-3′; and other sequences. |
| hrGFP | primers: 5′-gatccgcagcgacatcaacc-3′ and 5′-gtacaccacctcgaagctgg-3′; probe: R-5′-gaggagatgttcgtgtaccgcgtgg-3′-Q. | |
| emGFP | primers: 5′-acggcgacgtaaacggccac-3′ and 5′-gcgaagcactgcacgccgta-3′. | |
| pU6 | primers: 5′-gggaaataggccctcttcctgccc-3′ and 5′-caccacgtgacggagcgtgac-3′. | |
| ITR5 | primers: 5′-cccccccaaacgagccag-3′ and 5′-acccccttgcttgagag-3′; probe: R-5′- cgagcgaacgcgacaggggggagagtg-3′-Q. |
R—probe reporter, Q—probe quencher, BGH—bovine growth hormone, CAG—cytomegalovirus enhancer/chicken β-actin, CBA—chicken β-actin, CMV—Cytomegalovirus, eGFP—enhanced green fluorescent protein, emGFP—emerald green fluorescent protein, GRK1—rhodopsin kinase, hrGFP—humanized Renilla reniformis green fluorescent protein, ITR—inverted terminal repeat, polyA—polyadenylation signal, pU6—U6 promoter, RBG—rabbit β-globin, SV40—Simian virus 40, TBG—human thyroxine-binding globulin and WPRE—woodchuck hepatitis B virus posttranscriptional regulator