- Molecular Biology Grade Water, Hyclone, SH30538.02
- GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129
- ddPCR Supermix for Probes no dUTP, Bio-Rad,1863023
- 10% Pluronic F-68, Thermo Fisher, 24040032
- Droplet generation oil, Bio-Rad, 1863005
- DG32 cartridge, Bio-Rad, 1864108
- ddPCR 96-well PCR plates, Bio-Rad, 12001925
- Pierceable Foil Heat Seal, Bio-Rad, 1814040
- Polystyrene Reservoirs, VWR, 89094-662
- Microcentrifuge tubes, VWR, 87003-294
- Primers/probe targeting ITR:
- ITR Forward Primer: 5’-CGGCCTCAGTGAGCGA
- ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT
- ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ-