A single HEK293T cell can produce a wide range of adeno-associated virus (AAV) particles, typically in the range of 10,000 to 100,000 vector genomes per cell. The exact number varies depending on experimental conditions, including transfection efficiency, cell density, and harvesting time. While some sources suggest a range of 10,000 to 100,000 viral particles per cell, others specify yields of 5 x 10^4 to 3.5 x 10^5 vector genomes per cell for triple transfection methods. 

Here’s a more detailed breakdown:

• Typical range: A common estimate is that one HEK293T cell can produce between 10,000 and 100,000 AAV particles (or vector genomes). 
• Factors affecting yield: Several factors can influence the number of AAV particles produced, including:
  • Transfection efficiency: The effectiveness of introducing the necessary plasmids into the cells significantly impacts production. 
  • Cell density and health: Cells that are healthy and at the appropriate density tend to produce more virus. 
  • Harvesting time: The timing of harvest after transfection affects the number of viral particles released. 
  • Vector system and serotype: Different AAV serotypes and vector designs can have varying production yields.