| DNA Impurity | Target | Sequences |
| Plasmid | KanR | primers: 5′-gggcgcccggttctttttgtc-3′ and 5′-gccagtcccttcccgcttcagtg-3′. |
| AmpR | primers: 5′-cgcgccacatagcagaactt-3′ and 5′-cgccccgaagaacgttt-3′; probe: R-5′-aaaagtgctcatcattg-3′-Q; and other sequences. | |
| Adenovirus E2A | primers: 5′-ttgctgaaacccaccatttg-3′ and 5′-tcgtggacagcgaggaaga-3′; probe: R-5′-cgccacatcttctct-3′-Q. | |
| Adenovirus E4 | primers: 5′-tcggcgcactccgtaca-3′ and 5′-cgcgggtctctgtctcaaaa-3′; probe: R-5′-tagggatcgcctacctc-3′-Q. | |
| HSV UL23 | primers: 5′-tcgatgtgtctgtcctccg-3′ and 5′-atcccatcgccgccctc-3′. | |
| HSV UL24 | primers: 5′-gccgcgagaacgcgcag-3′ and 5′-cctcgaataccgagcgacc-3′. | |
| HSV UL26.5 | primers: 5′-catgtccttccacccagac-3′ and 5′-cccatcatctgagagacgaa-3′; probe: R-5′-cagcacacgtggacgttgacac-3′-Q. | |
| HSV UL29 | primers: 5′-ccgcctatggttaccttgtc-3′ and 5′-ccctcctgtatctggtcgtt-3′; probe: R-5′-agcctcccaggtgcagaaaggt-3′-Q. | |
| HSV UL33 | primers: 5′- cgaactttacgggacacaatc-3′ and 5′-cgtagtcgggaagacaacct-3′; probe: R-5′-tagacgcgcgctacgtctcg-3′-Q. | |
| HSV UL35 | primers: 5′-acgcaaacaacacgtttacc-3′ and 5′-tcgaaggttctcgaacgac-3′; probe: R-5′-cggcgcacctattcaccgttt-3′-Q. | |
| Ori | primers: 5′-gcgcgtaatctgctgcttg-3′ and 5′-ctacggctacactagaagaacagta-3′; probe: R-5′-cgctctgctgaagccagttaccttcgg-3′-Q. | |
| cap8 | primers: 5′-tcagccaaggtgggcctaatacaa-3′ and 5′-ttgctgctgcaagttatctgccac-3′; and other sequences. | |
| rep2 | primers: 5′-cggggttttacgagattgtg-3′ and 5′-cgccatttctggtctttgtg-3′. | |
| cap2 | primers: 5′-ttctcagatgctgcgtaccggaaa-3′ and 5′-tctgccattgaggtggtacttggt-3′. | |
| Host cell DNA | human Alu | primers: 5′-gaggcgggcggatca-3′ and 5′-cccggctaatttttgtatttttagtag-3′; probe: R-5′-cagcctggccaacatggtgaaacc-3′-Q; and other sequences. |
| Adenovirus E1A | primers: 5′-gggtgaggagtttgtgttagattatg-3′ and 5′-tcctccggtgataatgacaaga-3′; probe: R-5′-agcaccccgggcacggttg-3′-Q. | |
| chinese hamster Alu | primers: 5′-agagatggctcgaggttaag-3′ and 5′-tctgcacaccagaagagg-3′; probe: R-5′-agcaccaactgctcttccagagg-3′-Q. | |
| Syrian hamster 5S rRNA | primers: 5′-cgcagcagcaggctct-3′ and 5′-accctgcttagcttccgaga-3′; probe: R-5′-ccgccgtcgtctacggccatacc-3′-Q. | |
| rcAAV | ITR-Rep recombinants | primers: 5′-actccatcactaggggttct-3′ and 5′-gctggggaccttaatcacaa-3′. |
R—probe reporter, Q—probe quencher and HSV—herpes simplex virus.