Summary

In 2009 Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear DNA fragments (Nat Methods 2009;6(5):343-5). Regardless of fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single isothermal reaction. With the activities of three different enzymes, the product of a Gibson Assembly is a fully ligated double-stranded DNA molecule. This has proven to be an efficient and effective method for the assembly of plasmids, and molecular biologists now use this method extensively.

Why Gibson Cloning?

• No need for specific restriction sites. Join almost any 2 fragments regardless of sequence.
• No scar between joined fragments.
• Fewer steps. One tube reaction.
• Can combine many DNA fragments at once.