Product Name | Gram (+)bacteria | Gram (-)bacteria | Yeasts | Molds | Mycoplasma | Suggested Working Conc. |
Amphotericin B | † | † | 2.5 mg/L | |||
Amphotericin B-Solubilized (Approx. 45%) | † | † | 5.6 mg/L (of solid) | |||
Amphotericin B(250 μg/ml solution) | † | † | 10 ml/L | |||
Ampicillin | † | † | 100 mg/L | |||
Antibiotic Antimycotic Solution (100x)(10,000 units penicillin, 10 mg streptomycin, and 25 µg amphotericin B per ml) | † | † | † | † | 10 ml/L | |
Cephalothin | † | † | 100 mg/L | |||
Dihydrostreptomycin | † | † | 100 mg/L | |||
Erythromycin | † | † | 100 mg/L | |||
Gentamicin Sulfate | † | † | † | 50 mg/L | ||
Gentamicin Sulfate (10 mg/ml solution) | † | † | † | 5 ml/L | ||
Gentamicin Sulfate (50 mg/ml solution) | † | † | † | 1 ml/L | ||
L-Glutamine-Penicillin-Streptomycin Solution (200 mM L- glutamine, 10,000 units penicillin, and 10 mg streptomycinper ml) | † | † | 10 ml/L | |||
Kanamycin Monosulfate | † | † | † | 100 ml/L | ||
Kanamycin Sulfate (10 mg/ml solution) | † | † | † | 10 ml/L | ||
Kanamycin Sulfate (50 mg/ml solution) | † | † | † | 2 ml/L | ||
Lincomycin HCI | † | 100 mg/L | ||||
Neomycin Sulfate | † | † | 50 mg/L | |||
Neomycin Sulfate (10 mg/ml solution) | † | † | 5 ml/L | |||
Nystatin (5,000 units nystatin per mg) | † | † | 2.5 X 105U/L (50 mg/L) | |||
Nystatin | † | † | 50 mg/L | |||
Nystatin Suspension | † | † | 24 ml/L | |||
Paromomycin Sulfate | † | † | 100mg mg/L | |||
Penicillin-G (potassium salt) | † | 100,00 U/L | ||||
Penicillin-G (sodium salt) | † | 100,000 U/L | ||||
Penicillin-Streptomycin solution (5,000 units penicillin-G and 5 mg streptomycin per ml) | † | † | 20 ml/L | |||
Penicillin-Streptomycin Solution (10,000 units penicillin-G and 10 mg streptomycin per ml) | † | † | 10 mg/L | |||
Penicillin-Streptomycin-Neomycin solution (5,000 units penicillin-G,5 mg streptomycin and 10 mg neomycin per ml) | † | † | 10 ml/L | |||
Phenoxymethylpenicillinic Acid (potassium salt) [Penicillin V] | † | 100,000 U/L | ||||
Polymyxin B Sulfate | † | 50 mg/L | ||||
SpectinomycinDihydrochloride | † | † | 7.5-20 mg/L | |||
Spectromycin Sulfate | † | † | 10 mg/L | |||
TetracyclineHydrochloride | † | † | 10 mg/L | |||
Tylosin Tartrate(8 mg/ml soln.) | † | † | 1 ml/L | |||
ANTIBIOTICS FOR PLANT CELL CULTURE | ||||||
Product Name | Gram (+)bacteria | Gram (-)bacteria | Mycobacteria | Yeasts | Molds | Mycoplasma |
Carbenicillin | ‡ | † | ||||
Cefotaxime | ‡ | † | ||||
Chloramphenicol | † | † | ‡ | ‡ | ||
G 418 | ||||||
Gentamicin Sulfate | ‡ | † | † | |||
Hygromycin B | ||||||
Kanamycin | † | † | † | |||
Paromycin | † | ‡ | ||||
Penicillin G, Potassium | † | ‡ | ||||
Penicillin G, Sodium | † | ‡ | ||||
Rifampicin | † | † | † | |||
Streptomycin Sulfate | ‡ | † | ‡ | |||
Vancomycin | † | |||||
Product Name | Gram (+)bacteria | Gram (-)bacteria | Myco-bacteria | Yeasts | Molds | Myco-plasma |
Nystatin | † | † | ||||
Pentachloronitrobenzene | † | |||||
Thiabendazole | † | |||||
† = Effective against most species | ||||||
‡ = Effective against certain species | ||||||
1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant | ||||||
selection; SE = somatic embryogenesis; SSC = stem selection culture; PC = protoplast culture; TEC = tuber explant culture; | ||||||
STM = shoot tip micropropagation | ||||||
ANTIBIOTIC SELECTION AGENTS FOR CELL CULTURE | ||||||
Product Name | Mode of Action | Suggested Working Conc. | ||||
Actinomycin D | Complexes with DNA and interferes with RNA synthesis | 1 µg/ml | ||||
Bleomycin Sulfate | Complexes with DNA, causing strand scissions | 10-100 µg/ml | ||||
Chloramphenicol | Inhibits elongation at peptidyl transferase | 5 µg/ml | ||||
G 418 | Blocks polypeptide synthesis and inhibits chain elongation | 100-800 µg/ml | ||||
G 418 (50 mg/ml solution) | Blocks polypeptide synthesis and inhibits chain elongation | 1 µg/ml | ||||
Hygromycin B | Blocks polypeptide synthesis and inhibits chain elongation | |||||
Mitomycin C | Inhibits nucleic acid synthesis | 10-50 µg/ml | ||||
Mycophenolic Acid | Blocks inosine monophosphate dehydrogenase in guanosine monophosphate pathway | 25 µg/ml | ||||
Puromycin HCl | Inhibits protein synthesis | 10-100 µg/ml |