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Primers for rAAV qPCR Titration

Cytomegalovirus (CMV) Promoter Assay
Forward: 5-CATCTACGTATTAGTCATCGCTATTACCA-3
Reverse: 5-GAAATCCCCGTGAGTCAAACC-3
Probe: 5-6FAM-TCAATGGGCGTGGATAG-MGBNFQ-3

Human Thyroxine-binding Globulin (TBG) Promoter Assay
Forward: 5-AAACTGCCAATTCCACTGCTG-3
Reverse: 5-CCATAGGCAAAAGCACCAAGA-3
Probe: 5-6FAM-TTGGCCCAATAGTGAGAACTTTTTCCTGC-TAMRA-3

Enhanced Green Fluorescent Protein (eGFP) Assay
Forward: 5-AGCAAAGACCCCAACGAGAA-3
Reverse: 5-GGCGGCGGTCACGAA-3
Probe: 5-6FAM-CGCGATCACATGGTCCTGCTGG-TAMRA-3

Bovine Growth Hormone (BGH) Polyadenylation Signal Assay
Forward: 5-GCCAGCCATCTGTTGT-3
Reverse: 5-GGAGTGGCACCTTCCA-3
Probe: 5-6FAM-TCCCCCGTGCCTTCCTTGACC-TAMRA-3
Forward: 5’-GCCTTCTAGTTGCCAGCCAT-3’
Reverse: 5’-GGCACCTTCCAGGGTCAAG-3’

Normal Rabbit beta-globin (nRBG) Polyadenylation Signal Assay
Forward: 5-GCCAAAAATTATGGGGACAT-3
Reverse: 5-ATTCCAACACACTATTGCAATG-3
Probe: 5-6FAM-ATGAAGCCCCTTGAGCATCTGACTTCT-TAMRA-3

Simian Virus 40 (SV40) Polyadenylation Signal Assay
Forward: 5-AGCAATAGCATCACAAATTTCACAA-3
Reverse: 5-CCAGACATGATAAGATACATTGATGAGTT-3
Probe: 5-6FAM-AGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTC-TAMRA-3

eYFP
Forward (5’-CCACCTACGGCAAGCTGACC)
Reverse (5’-GGTAGCGGGCGAAGCACT)

mCherry
Forward (5’-GAACGGCCACGAGTTCGAGA)
Reverse (5’-CTTGGAGCCGTACATGAACTGAGG).

 

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