• A, B Representative 20× confocal images of immunostained vibratome sections from retinas of mice subretinally injected (A) or intravitreally injected (B) with viruses. The upper panels show AAV2/8(EF1α-EGFP) injected retinas, and the lower panels show AAV2/8BP2(EF1α-EGFP) injected retinas. The retinas are stained for EGFP (green), cell nuclei (gray), and for the inner plexiform layer strata using choline acetyltransferase (ChAT) (magenta). POS, photoreceptor outer segments; ONL, outer nuclear layer; OPL, outer plexiform layer; IPL, inner plexiform layer; GCL, ganglion cell layer.
• C The number of genome copies per cell was estimated for purified AAV2/8BP2 compared to AAV2/8 following transduction of HEK293 cells.
• D Cell counts from FACS analysis of retinas from mice (n = 4) subretinally injected with AAV2/8(EF1α-EGFP) or AAV2/8BP2(EF1α-EGFP).
• E RT-qPCR on RNA from the sorted cells used to determine bipolar cell gene expression levels with 120% increase in Grm6 expression (P = 0.05) and 67% increase in TrpM1L expression (P = 0.04) in the AAV2/8BP2(EF1α-EGFP) cell pool.
• F Equivalent expression levels measured between cell pools for the cone photoreceptor genes Opnmwl and Opnswl.
• G Representative 40× confocal images of sections from the retinas of mice subretinally injected with AAV2/8(4 × GRM6-EGFP) (upper panel) and AAV2/8BP2(4 × GRM6-EGFP) (lower panel). The panel on the left shows sections stained for EGFP (green) and cell nuclei (blue), while the panel on the right shows live fluorescence images.
• H Retinas from mice that were intravitreally injected were similarly analyzed.

Refer to EMBO Mol Med. 2014 Aug 4;6(9):1175-90. doi: 10.15252/emmm.201404077