All the following pre-made AAVs were packaged in serotype 1**, 2, 5, 6, 8** and 9** and super purified via 2xCsCl ultra-centrifugation in in-vivo grade. All the pre-packaged rAAVs were […]
Daily Archives: October 12, 2015
Summary of clinical trials using AAV gene transfer
Disease Transgene product Serotype Route of administration Clinical trial ClinicalTrials.gov identifier Refs AAV clinical trials for inherited diseases α1 antitrypsin deficiency α1 antitrypsin AAV2 Intramuscular Phase I/II NCT00377416 101,102 AAV1 […]
Key properties of a viral vector
Viral vectors are tailored to their specific applications but generally share a few key properties: Safety: Although viral vectors are occasionally created from pathogenic viruses, they are modified in such […]
Pre-made Lentivirus from GeneCopoeia
Positive and negative controls: eGFP eYFP eCFP mCherry PLUM Firefly luciferase Renilla luciferase Gaussia luciferase Firefly luciferase + eGFP/mCherry Renilla luciferase + eGFP/mCherry Firefly luciferase + IRES-mCherry Renilla luciferase + […]
mRFP expression in neurones and glia in dorsal vagal and hypoglossal motor nuclei
Representative images are shown for the experimental conditions evaluated. mRFP-expressing cells transduced with either AVV- or LVV-CMV-mRFP are shown in the left hand panels; IR for NeuN or GFAP in […]
WHAT ARE SOME DIFFERENT WAYS TO TITER LENTIVIRUS?
Functional titer is defined as the number of functional vector particles required to infect a cell, present in a volume. There are several methods for measuring titer. Some are more […]
PRACTICAL APPLICATIONS AND LIMITATIONS OF LENTIVIRAL VECTOR
Applications of lentivirus vectors include stable gene transfer for gene delivery in gene and cell therapy. This includes using RNA interference technology to block the expression of specific genes. Along […]
WHAT IS PSEUDOTYPING of LENTIVIRUS?
In HIV, pseudotyping is used to change the tropism of the virion. In other words, pseudotyping changes the way the Lentivector targets a specific cell for infection. In wild type […]
WHAT MAKES LENTIVIRUS SO SPECIAL?
Though there are several virus delivery systems available today for gene therapy, lentivirus is considered one of the best methods. Several features make lentivirus preferable especially the ability to infect […]
Overview of Lentivirus Pseudotypes
Family Genus Species Vector References Rhabdoviridae Vesiculovirus Vesicular stomatitis virus (Indiana virus) HIV-1 [Akkina, 1996][Naldini, 1996][Reiser, 1996] HIV-2 [Poeschla, 1998a] FIV [Poeschla, 1998b] EIAV [Olsen, 1998] BIV [Berkowitz, 2001a] JDV […]