Pre-made AAV, AAV2-CAG-ChIEF-tdTOMATO
Product Category:
Pre-made recombinant
AAV;
AAV-CAG-ChIEF-tdTOMATO;
AAV2-CAG-ChIEF-tdTOMATO
Description:
AAV2-ChIEF-tdTOMATO the serotype 2 rAAV which expresses a hybrid of Channelrhodopsin-1/2 under CAG
promoter fusion with reporter tdTOMATO.
ChIEF is a hybrid of channelrhodopsin 1 and 2 with additional L170I mutation
(ChR1 numbering) or at L131I (ChR2-numbering), leading to large currents in
oocytes and HEK-cells and almost wild type like open state life time.
ChIEF function as light-gated ion channels and very useful for many
bioengineering and neuroscience applications such as photostimulation of neurons
for probing of neural circuits. Using tdTOMATO tagged ChIEF, light-stimulated
axons and synapses can be identified in intact brain tissue. This is useful to
study the molecular events during the induction of synaptic plasticity. ChIEF
has also been used to map long-range connections from one side of the brain to
the other, and to map the spatial location of specific inputs on the dendritic
tree of individual neurons.
Titer:
> 1x1013 VG/mL
Size: 30 µL
(Std Pack)
Storage Buffer:
Sterile PBS
Infection Protocol:
Vector Map:
