Product Category: Pre-made
recombinant lentivirus;
LV-EF1a-Mito-DsRed-IRES-Puro; LV-EF1a-Mito-DsRed-Puro
Description: LV-EF1a-Mito-DsRed-IRES-Puro
is a pre-made lentivirus which expresses Mito-EDsRed under the EF1a promoter wth
the co-expression of the Puro resistance marker via the IRES element. The
mitochondrial targeting sequence from subunit VIII of human cytochrome C
oxidase (Mito) is fused with EDsRed. The Mito sequence is fused to the 5'-end of
EDsRed, The Mito sequence targets the Mito-EDsRed fusion protein to the host
cell’s mitochondria.
Titer: >
1x109 TU/mL
Selection Antibiotic: Puromycin
Purity*: In
vivo grade and OK for both in vitro tissue culture infection and in vivo
injection
Size: 2
x 25 µL (Std
Pack)
Storage Buffer: PBS
Infection Protocol:
Transfer Vector Map:
* Concentrated
via PEG precipitation followed by
ultra-centrifugation at 25000 rpm