LV-CMV-SpCas9

Product Category: Pre-made recombinant lentivirus; LV-CMV-SpCas9; LV-CMV-Cas9; CMV-Cas9 Lentivirus

Description: LV-CMV-SpCas9 is a pre-made lentivirus which over-expresses SpCas9 under the CMV promoter and can be used along with guided RNA for genome-editing. The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering.  Cas9 (CRISPR associated protein 9) is an RNA-guided DNA nuclease enzyme associated with Streptococcus pyogenes' CRISPR immunity system.  The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.   Ready to use format. 

Titer: > 1x10⁹ TU/mL

Selection Antibiotic: None

Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection

Size: 2 x 25 µL (Std Pack)

Storage Buffer: PBS

Infection Protocol: 

Transfer Vector Map:

* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm