Product Category: Pre-made
recombinant lentivirus; LV-CAG-FLPo; LV-CBA-FLPo; CAG-FLPo Lentivirus;
CBA-FLPo Lentivirus
Description: FLP
originally identified from Saccharomyces cerevisiae is a site-specific
recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp
FRT sequence. It has been widely used for generating conditional knockout
mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells
revealed inefficient recombinase activity due to thermo-instability of the FLP
protein. Subsequent screening for thermo-stable mutants resulted in the
identification of FLPe which was 4-fold increase in recombination efficiency
at 37 °C. However the recombination efficiency of FLPe in cells remains very
low because of its non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT
recombination efficiency for both in vivo and in vitro in mouse. The
LV-CAG-Puro
is a
pre-made lentivirus which
over-expresses the FLPo recombinase upon infection of the mammalian cells.
Ready to use format.
Titer: >
1x109 TU/mL
Selection Antibiotic: None
Purity*: In
vivo grade and OK for both in vitro tissue culture infection and in vivo
injection
Size: 2 x 25 µL
(Std Pack)
Storage Buffer: PBS
Infection Protocol: 
Transfer Vector Map:
* Concentrated
via PEG precipitation followed by
ultra-centrifugation at 28000 rpm
