Pre-made human adenovirus serotype 5 (dE1/E3), FLPo recombinase adenovirus; Ad-CMV-FLPo; Ad-FLPo
Product Category: Pre-made human
adenovirus serotype 5 (dE1/E3), FLPo recombinase adenovirus; Ad-CMV-FLPo; Ad-FLPo
Description: FLP
originally identified from Saccharomyces cerevisiae is a site-specific
recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT
sequence. It has been widely used for generating conditional knockout mice,
mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed
inefficient recombinase activity due to thermo-instability of the FLP protein.
Subsequent screening for thermo-stable mutants resulted in the identification of
FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the
recombination efficiency of FLPe in cells remains very low because of its
non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT
recombination efficiency for both in vivo and in vitro in mouse. This
Ad-CMV-FLPo
pre-packaged adenovirus
over-expresses FLPo recombinase under
CMV promoter. Ready
to use format.
Titer: 1x1010 ~ 1x1011
PFU/mL
Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol
User Manual:
Shuttle Vector Map:
